E8906
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 | Immunoblotting Rat PC12 whole cell extract was separated on SDS-PAGE and probed with Rabbit Anti-EDEM3 (Cat. No. E8906). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 5 μg/mL 2. Antibody 5 μg/mL + EDEM3 immunizing peptide (human, 470-485) |
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E8906
Sigma
Anti-EDEM3 antibody produced in rabbit
~1.2 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-ER degradation enhancer, mannosidase alpha-like 3 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 470-485 of human EDEM3, conjugated to KLH. The corresponding sequence is identical in mouse and rat EDEM3. |
| Physical form | Solution in 0.01 M phosÂphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~120 kDa |
| species reactivity | rat, human, mouse |
| concentration | ~1.2 mg/mL |
| application(s) | immunoblotting: 2-5 μg/mL using whole extract of rat PC12 cells |
| immunoprecipitation: 2-5 μg using mouse 3T3 and human HepG2 cell lysates | |
| Gene Information | human ... EDEM3(80267) |
| mouse ... Edem3(66967) | |
| shipped in | dry ice |
| storage temp. | −20°C |
